Characterization of novel monoclonal antibodies against the MERS-coronavirus spike protein and their application in species-independent antibody detection by competitive ELISA
Identifieur interne : 000E65 ( Main/Exploration ); précédent : 000E64; suivant : 000E66Characterization of novel monoclonal antibodies against the MERS-coronavirus spike protein and their application in species-independent antibody detection by competitive ELISA
Auteurs : Shuetsu Fukushi [Japon] ; Aiko Fukuma [Japon] ; Takeshi Kurosu [Japon] ; Shumpei Watanabe [Japon] ; Masayuki Shimojima [Japon] ; Kazuya Shirato [Japon] ; Naoko Iwata-Yoshikawa [Japon] ; Noriyo Nagata [Japon] ; Kazuo Ohnishi [Japon] ; Manabu Ato [Japon] ; Simenew Keskes Melaku [Éthiopie] ; Hiroshi Sentsui [Japon] ; Masayuki Saijo [Japon]Source :
- Journal of Virological Methods [ 0166-0934 ] ; 2017.
Descripteurs français
- KwdFr :
- Animaux, Anticorps antiviraux (immunologie), Anticorps antiviraux (isolement et purification), Anticorps monoclonaux (immunologie), Anticorps monoclonaux (isolement et purification), Anticorps monoclonaux (sang), Chameaux, Coronavirus du syndrome respiratoire du Moyen-Orient (immunologie), Glycoprotéine de spicule des coronavirus (immunologie), Lapins, Rats, Sensibilité et spécificité, Test ELISA ().
- MESH :
- immunologie : Anticorps antiviraux, Anticorps monoclonaux, Coronavirus du syndrome respiratoire du Moyen-Orient, Glycoprotéine de spicule des coronavirus.
- isolement et purification : Anticorps antiviraux, Anticorps monoclonaux.
- sang : Anticorps monoclonaux.
- Animaux, Chameaux, Lapins, Rats, Sensibilité et spécificité, Test ELISA.
English descriptors
- KwdEn :
- Animals, Antibodies, Monoclonal (blood), Antibodies, Monoclonal (immunology), Antibodies, Monoclonal (isolation & purification), Antibodies, Viral (immunology), Antibodies, Viral (isolation & purification), Camelus, Enzyme-Linked Immunosorbent Assay (methods), Middle East Respiratory Syndrome Coronavirus (immunology), Rabbits, Rats, Sensitivity and Specificity, Spike Glycoprotein, Coronavirus (immunology).
- MESH :
- chemical , blood : Antibodies, Monoclonal.
- chemical , immunology : Antibodies, Monoclonal, Antibodies, Viral, Spike Glycoprotein, Coronavirus.
- chemical , isolation & purification : Antibodies, Monoclonal, Antibodies, Viral.
- immunology : Middle East Respiratory Syndrome Coronavirus.
- methods : Enzyme-Linked Immunosorbent Assay.
- Animals, Camelus, Rabbits, Rats, Sensitivity and Specificity.
Abstract
Novel monoclonal antibodies against MERS-CoV were produced and characterized. Anti-MERS-CoV antibody detection system by competitive ELISA was developed. The competitive ELISA was validated using sera taken from dromedary camels.
Url:
DOI: 10.1016/j.jviromet.2017.10.008
PubMed: 28993122
PubMed Central: 7113858
Affiliations:
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sortKey="Fukushi, Shuetsu" sort="Fukushi, Shuetsu" uniqKey="Fukushi S" first="Shuetsu" last="Fukushi">Shuetsu Fukushi</name><affiliation wicri:level="1"><nlm:aff id="aff0005">Department of Virology I, National Institute of Infectious Diseases, Japan</nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Virology I, National Institute of Infectious Diseases</wicri:regionArea><wicri:noRegion>National Institute of Infectious Diseases</wicri:noRegion></affiliation></author><author><name sortKey="Fukuma, Aiko" sort="Fukuma, Aiko" uniqKey="Fukuma A" first="Aiko" last="Fukuma">Aiko Fukuma</name><affiliation wicri:level="1"><nlm:aff id="aff0005">Department of Virology I, National Institute of Infectious Diseases, Japan</nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Virology I, National Institute of Infectious Diseases</wicri:regionArea><wicri:noRegion>National Institute of Infectious Diseases</wicri:noRegion></affiliation></author><author><name sortKey="Kurosu, Takeshi" sort="Kurosu, Takeshi" uniqKey="Kurosu T" first="Takeshi" last="Kurosu">Takeshi Kurosu</name><affiliation wicri:level="1"><nlm:aff id="aff0005">Department of Virology I, National Institute of Infectious Diseases, Japan</nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Virology I, National Institute of Infectious Diseases</wicri:regionArea><wicri:noRegion>National Institute of Infectious Diseases</wicri:noRegion></affiliation></author><author><name sortKey="Watanabe, Shumpei" sort="Watanabe, Shumpei" uniqKey="Watanabe S" first="Shumpei" last="Watanabe">Shumpei Watanabe</name><affiliation wicri:level="1"><nlm:aff id="aff0005">Department of Virology I, National Institute of Infectious Diseases, Japan</nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Virology I, National Institute of Infectious Diseases</wicri:regionArea><wicri:noRegion>National Institute of Infectious Diseases</wicri:noRegion></affiliation></author><author><name sortKey="Shimojima, Masayuki" sort="Shimojima, Masayuki" uniqKey="Shimojima M" first="Masayuki" last="Shimojima">Masayuki Shimojima</name><affiliation wicri:level="1"><nlm:aff id="aff0005">Department of Virology I, National Institute of Infectious Diseases, Japan</nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Virology I, National Institute of Infectious Diseases</wicri:regionArea><wicri:noRegion>National Institute of Infectious Diseases</wicri:noRegion></affiliation></author><author><name sortKey="Shirato, Kazuya" sort="Shirato, Kazuya" uniqKey="Shirato K" first="Kazuya" last="Shirato">Kazuya Shirato</name><affiliation wicri:level="1"><nlm:aff id="aff0010">Department of Virology III, National Institute of Infectious Diseases, Japan</nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Virology III, National Institute of Infectious Diseases</wicri:regionArea><wicri:noRegion>National Institute of Infectious Diseases</wicri:noRegion></affiliation></author><author><name sortKey="Iwata Yoshikawa, Naoko" sort="Iwata Yoshikawa, Naoko" uniqKey="Iwata Yoshikawa N" first="Naoko" last="Iwata-Yoshikawa">Naoko Iwata-Yoshikawa</name><affiliation wicri:level="1"><nlm:aff id="aff0015">Department of Pathology, National Institute of Infectious Diseases, Japan</nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Pathology, National Institute of Infectious Diseases</wicri:regionArea><wicri:noRegion>National Institute of Infectious Diseases</wicri:noRegion></affiliation></author><author><name sortKey="Nagata, Noriyo" sort="Nagata, Noriyo" uniqKey="Nagata N" first="Noriyo" last="Nagata">Noriyo Nagata</name><affiliation wicri:level="1"><nlm:aff id="aff0015">Department of Pathology, National Institute of Infectious Diseases, Japan</nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Pathology, National Institute of Infectious Diseases</wicri:regionArea><wicri:noRegion>National Institute of Infectious Diseases</wicri:noRegion></affiliation></author><author><name sortKey="Ohnishi, Kazuo" sort="Ohnishi, Kazuo" uniqKey="Ohnishi K" first="Kazuo" last="Ohnishi">Kazuo Ohnishi</name><affiliation wicri:level="1"><nlm:aff id="aff0020">Department of Immunology, National Institute of Infectious Diseases, Japan</nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Immunology, National Institute of Infectious Diseases</wicri:regionArea><wicri:noRegion>National Institute of Infectious Diseases</wicri:noRegion></affiliation></author><author><name sortKey="Ato, Manabu" sort="Ato, Manabu" uniqKey="Ato M" first="Manabu" last="Ato">Manabu Ato</name><affiliation wicri:level="1"><nlm:aff id="aff0020">Department of Immunology, National Institute of Infectious Diseases, Japan</nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Immunology, National Institute of Infectious Diseases</wicri:regionArea><wicri:noRegion>National Institute of Infectious Diseases</wicri:noRegion></affiliation></author><author><name sortKey="Melaku, Simenew Keskes" sort="Melaku, Simenew Keskes" uniqKey="Melaku S" first="Simenew Keskes" last="Melaku">Simenew Keskes Melaku</name><affiliation wicri:level="1"><nlm:aff id="aff0025">Department of Biotechnology, College of Biological and Chemical Engineering, Addis Ababa Science and Technology University, Ethiopia</nlm:aff><country xml:lang="fr">Éthiopie</country><wicri:regionArea>Department of Biotechnology, College of Biological and Chemical Engineering, Addis Ababa Science and Technology University</wicri:regionArea><wicri:noRegion>Addis Ababa Science and Technology University</wicri:noRegion></affiliation></author><author><name sortKey="Sentsui, Hiroshi" sort="Sentsui, Hiroshi" uniqKey="Sentsui H" first="Hiroshi" last="Sentsui">Hiroshi Sentsui</name><affiliation wicri:level="1"><nlm:aff id="aff0030">Department of Veterinary Medicine, Nihon University, Japan</nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Veterinary Medicine, Nihon University</wicri:regionArea><wicri:noRegion>Nihon University</wicri:noRegion></affiliation></author><author><name sortKey="Saijo, Masayuki" sort="Saijo, Masayuki" uniqKey="Saijo M" first="Masayuki" last="Saijo">Masayuki Saijo</name><affiliation wicri:level="1"><nlm:aff id="aff0005">Department of Virology I, National Institute of Infectious Diseases, Japan</nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Virology I, National Institute of Infectious Diseases</wicri:regionArea><wicri:noRegion>National Institute of Infectious Diseases</wicri:noRegion></affiliation></author></analytic><series><title level="j">Journal of Virological Methods</title><idno type="ISSN">0166-0934</idno><idno type="eISSN">1879-0984</idno><imprint><date 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monoclonaux (sang)</term><term>Chameaux</term><term>Coronavirus du syndrome respiratoire du Moyen-Orient (immunologie)</term><term>Glycoprotéine de spicule des coronavirus (immunologie)</term><term>Lapins</term><term>Rats</term><term>Sensibilité et spécificité</term><term>Test ELISA ()</term></keywords><keywords scheme="MESH" type="chemical" qualifier="blood" xml:lang="en"><term>Antibodies, Monoclonal</term></keywords><keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Antibodies, Monoclonal</term><term>Antibodies, Viral</term><term>Spike Glycoprotein, Coronavirus</term></keywords><keywords scheme="MESH" type="chemical" qualifier="isolation & purification" xml:lang="en"><term>Antibodies, Monoclonal</term><term>Antibodies, Viral</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Anticorps antiviraux</term><term>Anticorps monoclonaux</term><term>Coronavirus du syndrome respiratoire du Moyen-Orient</term><term>Glycoprotéine de spicule des coronavirus</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Middle East Respiratory Syndrome Coronavirus</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Anticorps antiviraux</term><term>Anticorps monoclonaux</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Enzyme-Linked Immunosorbent Assay</term></keywords><keywords scheme="MESH" qualifier="sang" xml:lang="fr"><term>Anticorps monoclonaux</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Camelus</term><term>Rabbits</term><term>Rats</term><term>Sensitivity and Specificity</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Chameaux</term><term>Lapins</term><term>Rats</term><term>Sensibilité et spécificité</term><term>Test ELISA</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><title>Highlights</title><p><list list-type="simple" id="lis0005"><list-item id="lsti0005"><label>•</label><p id="par0005">Novel monoclonal antibodies against MERS-CoV were produced and characterized.</p></list-item><list-item id="lsti0010"><label>•</label><p id="par0010">Anti-MERS-CoV antibody detection system by competitive ELISA was developed.</p></list-item><list-item id="lsti0015"><label>•</label><p id="par0015">The competitive ELISA was validated using sera taken from dromedary camels.</p></list-item></list></p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Al Hammadi, Z M" uniqKey="Al Hammadi Z">Z.M. Al Hammadi</name></author><author><name sortKey="Chu, D K" uniqKey="Chu D">D.K. Chu</name></author><author><name sortKey="Eltahir, Y M" uniqKey="Eltahir Y">Y.M. Eltahir</name></author><author><name sortKey="Al Hosani, F" uniqKey="Al Hosani F">F. Al Hosani</name></author><author><name sortKey="Al Mulla, M" uniqKey="Al Mulla M">M. Al Mulla</name></author><author><name sortKey="Tarnini, W" uniqKey="Tarnini W">W. Tarnini</name></author><author><name sortKey="Hall, A J" uniqKey="Hall A">A.J. Hall</name></author><author><name sortKey="Perera, R A" uniqKey="Perera R">R.A. Perera</name></author><author><name sortKey="Abdelkhalek, M M" uniqKey="Abdelkhalek M">M.M. Abdelkhalek</name></author><author><name sortKey="Peiris, J S" uniqKey="Peiris J">J.S. Peiris</name></author><author><name sortKey="Al Muhairi, S S" uniqKey="Al Muhairi S">S.S. Al Muhairi</name></author><author><name sortKey="Poon, L L" uniqKey="Poon L">L.L. 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Ziebuhr</name></author></analytic></biblStruct></listBibl></div1></back></TEI><affiliations><list><country><li>Japon</li><li>Éthiopie</li></country></list><tree><country name="Japon"><noRegion><name sortKey="Fukushi, Shuetsu" sort="Fukushi, Shuetsu" uniqKey="Fukushi S" first="Shuetsu" last="Fukushi">Shuetsu Fukushi</name></noRegion><name sortKey="Ato, Manabu" sort="Ato, Manabu" uniqKey="Ato M" first="Manabu" last="Ato">Manabu Ato</name><name sortKey="Fukuma, Aiko" sort="Fukuma, Aiko" uniqKey="Fukuma A" first="Aiko" last="Fukuma">Aiko Fukuma</name><name sortKey="Iwata Yoshikawa, Naoko" sort="Iwata Yoshikawa, Naoko" uniqKey="Iwata Yoshikawa N" first="Naoko" last="Iwata-Yoshikawa">Naoko Iwata-Yoshikawa</name><name sortKey="Kurosu, Takeshi" sort="Kurosu, Takeshi" uniqKey="Kurosu T" first="Takeshi" last="Kurosu">Takeshi Kurosu</name><name sortKey="Nagata, Noriyo" sort="Nagata, Noriyo" uniqKey="Nagata N" first="Noriyo" last="Nagata">Noriyo Nagata</name><name sortKey="Ohnishi, Kazuo" sort="Ohnishi, Kazuo" uniqKey="Ohnishi K" first="Kazuo" last="Ohnishi">Kazuo Ohnishi</name><name sortKey="Saijo, Masayuki" sort="Saijo, Masayuki" uniqKey="Saijo M" first="Masayuki" last="Saijo">Masayuki Saijo</name><name sortKey="Sentsui, Hiroshi" sort="Sentsui, Hiroshi" uniqKey="Sentsui H" first="Hiroshi" last="Sentsui">Hiroshi Sentsui</name><name sortKey="Shimojima, Masayuki" sort="Shimojima, Masayuki" uniqKey="Shimojima M" first="Masayuki" last="Shimojima">Masayuki Shimojima</name><name sortKey="Shirato, Kazuya" sort="Shirato, Kazuya" uniqKey="Shirato K" first="Kazuya" last="Shirato">Kazuya Shirato</name><name sortKey="Watanabe, Shumpei" sort="Watanabe, Shumpei" uniqKey="Watanabe S" first="Shumpei" last="Watanabe">Shumpei Watanabe</name></country><country name="Éthiopie"><noRegion><name sortKey="Melaku, Simenew Keskes" sort="Melaku, Simenew Keskes" uniqKey="Melaku S" first="Simenew Keskes" last="Melaku">Simenew Keskes Melaku</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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